Figure 2
From: High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells
HMGB1-induced EMT in human airway epithelial cells.
Human primary airway epithelial cells and BEAS-2B cells were treated with different doses of HMGB1 for mRNA analysis at different time points and at 24 h for protein analysis. E-cadherin, ZO-1 and vimentin mRNA expression was detected by real-time quantitative PCR in (a) human primary airway epithelial cells and (c) in BEAS-2B cells after HMGB1 treatment (300 ng/mL) for 24 h. E-cadherin, ZO-1 and vimentin protein expression in (b) human primary airway epithelial cells andBEAS-2B cells (d) was assessed by western blot analysis. Data are expressed as mean ± SD (n = 3–5). *p < 0.05, **p < 0.01, ***p < 0.001, as compared with the control group. (e) TGF-β1 expression in cell lysate (LAP) was assessed by western blot analysis and in cell medium (active form) by ELISA. Quantification of protein expression was performed using ImageJ software. Data are expressed as mean ± S.D. (n = 4). In immunoblotting assay, gels have been run under the same experimental conditions. Then cropped blots were incubated with different primary antibodies for analysis of EMT markers expression. Original blots of (b) are presented in Supplementary Fig. 3.
