Table 2 Effect of time and hairy roots presence on relative abundance of bacterial families present in horticultural growing media (n = 10).

Taxonomy	Time point	Growing medium		P value	Time effect	Time * hairy roots interaction
Taxonomy	Time point	RW (Mean ± SEM)	RWS (Mean ± SEM)	P value	Time effect	Time * hairy roots interaction
Hyphomicrobiaceae	1	1.53 ± 0.57	0.00	NS	0.01	NS
	2	4.47 ± 0.57	3.86 ± 0.57
	3	1.76 ± 0.57	1.68 ± 0.57
Methylophilaceae	1	1.54 ± 0.96	0.00	0.03	NS	NS
	2	4.09 ± 0.96^a	0.35 ± 0.96^b
	3	1.95 ± 0.96	0.005 ± 0.96
Planctomycetaceae	1	0.005 ± 0.05	0.00	0.0003	0.0007	0.0002
	2	0.02 ± 0.05^a	1.06 ± 0.05^b
	3	0.07 ± 0.05	0.01 ± 0.05
Rhizobiaceae	1	2.94 ± 0.85	0.00	0.0001	0.03	0.05
	2	1.02 ± 0.85^a	7.91 ± 0.85^b
	3	2.02 ± 0.8^a	13.02 ± 0.85^b
Verrucomicrobiaceae	1	0.57 ± 0.45	0.00	0.02	0.02	0.2
	2	0.98 ± 0.45^a	3.96 ± 0.45^b
	3	0.52 ± 0.45	0.53 ± 0.45
Vibrionaceae	1	0.16 ± 0.005	0.00	NS	< 0.0001	NS
	2	0.00	0.01 ± 0.005
	3	0.00	0.00

RW, mineral growing medium, n = 6. RWS, mineral growing medium with hairy roots, n = 4. NS = not significant effect. Different superscripts indicate significantly different means. “0.00” indicates zero relative abundance detected.

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