Figure 3

ORM1 specifically binds to the C-C Chemokine receptor type 5 (CCR5) in skeletal muscle.

(a) Representative immunofluorescence images of mouse primary skeletal muscle cells stained for FITC labelled BSA, FITC labelled ORM1 in absence or presence of 100 folds of non-FITC labelled ORM1 (n = 3). Nuclei were stained with DAPI (blue). (b) Representative flow cytometry analysis results of mouse primary skeletal muscle cells incubated as indicated in (a) and cells stained with blank were used as negative control (n = 3). (c) Representative histogram of U937 cells incubated with blank (negative control), FITC-labeled BSA, FITC-labeled ORM1 in the absence or presence of CCR5 antagonist Maraviroc or anti-CCR5 antibody. (d,e) Representative histogram of mouse primary skeletal muscle cells stained with blank (negative control), FITC-labeled BSA, FITC-labeled ORM1 in the absence or presence of Maraviroc (d) or anti-CCR5 antibody (e). Maraviroc: MVC. Skeletal muscle cell: SMC.