Figure 4

MicroRNA--target pairs supported by degradome signatures and comparison of expression patterns between the microRNAs and their targets.

(A) Comp155533_c0_seq1 and comp155533_c0_seq3 were predicted to be targeted by seven microRNAs (dof-miR-1017/1020/1021/1022/1023/1024/1034). The scatter diagram shows the degradome-seq data supporting the predicted regulation. Y axis measures the levels (in RPM, reads per million) of the degradome signatures and X axis represents the region surrounding the microRNA binding site (grey horizontal line). Compared with the signatures from the other organs, the most abundant one was identified from the flowers (arrowhead). The first line chart shows the levels (in RPKM, reads per kilobase of exon model per million mapped reads) of the two targets and the other two charts show the levels (in RPM) of three microRNAs (dof-miR-1017/1021/1034). Consistent with the degradome intensity, both the targets and the microRNAs are highly accumulated in the flowers (grey background color). (B) Comp156499_c0_seq2 was predicted to be targeted by seven microRNAs (dof-miR-985/986/987/988/989/990/991). The scatter diagram shows the degradome-seq data supporting the predicted regulation. Y axis measures the levels of the degradome signatures and X axis represents the region surrounding the predicted microRNA binding site (grey horizontal line). Compared with the signatures from the other organs, the most abundant one was identified from the leaves (arrowhead). The first line chart shows the level of the target and the other two show the levels of two microRNAs (dof-miR-988/989). Consistent with the degradome intensity, the two microRNAs are highly accumulated in the leaves (grey background color). (C) Comp126829_c0_seq1 was predicted to be targeted by dof-miR-1047. The scatter diagram shows the degradome-seq data supporting the predicted regulation. Y axis measures the levels of the degradome signatures. X axis represents the region surrounding the predicted microRNA binding site (grey horizontal line). Within this region, the degradome signals were only detectable in the flowers and the most abundant cleavage signal was denoted by an arrowhead. The first line chart shows the level of the target and the other chart shows the level of dof-miR-1047. Consistent with the degradome intensity, the target is highly expressed in the flowers (grey background color).