Figure 4
Confocal fluorescence imaging of exogenous sulphide in living MCF-7 cells using NIR-HS.
Cells were incubated with NIR-HS (5 μM) alone for 10 min (1A). Cells in panel 1A were thereafter treated with Na2S (50 μM) for 10 min (1B) and 20 min (1C). Cells were pretreated with 1 mM ZnCl2 for 10 min, then incubated with NIR-HS (5 μM) for 10 min and Na2S (50 μM) for 20 min (2A). Cells were incubated with NIR-HS (5 μM) for 10 min and then further incubated with Na2S (25 μM) for 20 min (2B). Scale bars = 10 μm. The average fluorescence intensity of the above images (2C). Data are presented as the mean ± SD (n = 3). #p < 0.001 vs. (1A) column, ##p < 0.001 vs. (1C) column.
