Figure 1

Different Chi levels in adjacent cells are essential for Notch signaling activity.

(a–a”’) Immunostaining of Chi (green) in wing discs of early third instar larvae. Cells with high Cut expression (red) locate in the boundary between high and low Chi protein regions. DAPI was used to mark the cell nuclei (blue). (b-b’) Immunostaining of Chi (red) in chi RNAi wing discs. chi RNAi was overexpressed in the clones (green). (c-f’) Immunostaining of Cut (c-d’; red) and Wg (e-f’; red) in yw and chi RNAi wing discs. (d’,f’) are the enlarged pictures of (d,f). Clones are marked with GFP (green). (g-h”) Immunostaining of Wg (g-g”) and Cut (h-h”) in the chi²⁶ wing discs. Wg (g; red) and Cut (h; red) expression are along the D/V boundary. Wg (g-g”) and Cut (h-h”) are activated in the boundary of chi²⁶ clones. (g’) and (h’) are the enlarged pictures of (g,h). Clones are marked with green and circled with dashed line. (i) Quantitative analysis of Cut activated cells around the clones. Shown are the Means ± s.d. (j) qPCR analysis of the Notch target genes. Shown are Means ± s.d. from 3 independent experiments. In every experiment, at least 20 discs were pooled together for analysis.