Figure 3
Chi and dLMO induce opposite functions on Notch signaling.
(a-b’) Immunostaining of Cut (a-a’; red) and Wg (b-b’; red) in the discs of bx RNAi flies. Cut and Wg activation did not occur at the clones (arrows) near A/P boundary. (a’,b’) are the enlarged pictures of (a,b). In all the IF results, clones are marked with GFP (green) and marked with dashed line. (c-d’) Immunostaining of Cut (c-c’; red) and Wg (d-d’; red) in the discs of bx overexpression flies. HA tag was marked with blue (d). (c’,d’) are the enlarged pictures of (c) and (d). (e-i’) Immunostaining of delta-lacZ (red) in the discs of indicated genotype flies. The delta was activated along the clone boundaries in the bx overexpression (e-e’) and chi loss-of-function flies (f-g’). The delta was downregulated inside the whole clones in the bx RNAi (h-h’) and chi overexpression flies (i-i’). (j-l’) Immunostaining of fng-lacZ (red) in the discs of indicated genotype flies. Compared to control (j-j’), fng was downregulated inside the whole clones in the chi26 (k-k’) and chi RNAi flies (l-l’).
