Figure 1 | Scientific Reports

Figure 1

From: Anti-inflammatory effects of indirubin derivatives on influenza A virus-infected human pulmonary microvascular endothelial cells

Figure 1

Infection of H9N2 on human pulmonary endothelial cells strongly induces expression of multiple cytokines.

HPMECs were infected with H9N2 at 2 MOI. Cell culture supernatant or total RNA were harvested at the indicated time point. The levels of (A) IP-10, (B) RANTES, (C) IL-6, (D) IL-1β, (E) MCP-1 and (F) TNF-α were measured by ELISA. The mRNA levels of (G) IFN-α1, (H) IFN-β and (I) IFN-γ1 were quantified by real-time RT-PCR. The values are presented as mean ± S.D. from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs mock-infected cells.

Back to article page