Figure 3 | Scientific Reports

Figure 3

From: Anti-inflammatory effects of indirubin derivatives on influenza A virus-infected human pulmonary microvascular endothelial cells

Figure 3

Indirubin derivatives E804 and E231 suppress H9N2-induced cytokines production in HPMECs in a concentration-related manner.

HPMECs were infected with H9N2 at 2 MOI followed by treatment with indirubin derivatives E804 or E231 (0.1, 1, 10 μM) for another 24 h. Cell culture supernatant and total RNA were harvested and the levels of (A) IP-10, (B) RANTES and (C) IL-6 were measured by ELISA. The mRNA levels of (D) IFN-β and (E) IFN-γ1 were quantified by real-time RT-PCR. The values are presented as mean ± S.D. from three independent experiments. **p < 0.01, ***p < 0.001 vs mock-infected cells. #p < 0.05, ##p < 0.01, ###p < 0.001 vs H9N2-infected cells.

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