Figure 4

Indirubin derivatives E804 and E231 do not affect H9N2 replication in human pulmonary endothelial cells.
HPMECs were infected with H9N2 at 2 MOI followed by treatment with indirubin derivatives E804 or E231 (1 μM) for another 24 h. (A) The mRNA level of influenza M1 gene was quantified by real-time RT-PCR. (B) The viral titers were quantified by standard plaque formation assay in MDCK cells. The values are presented as mean ± S.D. from three independent experiments. ***p < 0.001 vs mock-infected cells. ND = Not Detected.