Figure 5

H9N2-induced IP-10, RANTES and IL-6 productions in human pulmonary endothelial cells are mediated through MAPKs.

(A–C) HPMECs were infected with H9N2 at 2 MOI followed by treatment with either SP600125 (JNK inhibitors) (10 μM), SB203580 (p38 inhibitor) (10 μM), PD98059 (ERK inhibitor) (10 μM), indirubin derivatives E804 (1 μM) or E231 (1 μM) for another 24 h. (D–F) HPMECs transfected with non-targeting siRNA (50 nM) or STAT3 specific siRNA (50 nM) were infected with H9N2 at 2 MOI for 1 h and further incubated for 24 h.p.i. Cell culture supernatant was harvested and the levels of IP-10, RANTES and IL-6 were measured by ELISA. The values are presented as mean ± S.D. from three independent experiments. ***p < 0.001 vs mock-infected cells. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs H9N2-infected cells.