Figure 2
TGF-β1 increased TREM-1 expression in a time- and dose-dependent manner in mouse macrophages line.
TREM-1 mRNA expression (a) of mouse macrophages line following stimulation with 10 ng/mL TGF-β1 for various periods of time (0, 1, 2, 5, 10, 20 and 40 h) was examined by real-time PCR. TREM-1 protein level (b) on the surface of mouse macrophages line after stimulation with 10 ng/mL TGF-β1 for various periods of time was determined by Flow cytometry. TREM-1 mRNA expression (c) of macrophages line after incubation with TGF-β1 at various concentrations (0, 1, 2, 5, 10 and 20 ng/mL) for 20 h was detected by real-time PCR. The protein content of TREM-1 (d) on the surface of mouse macrophages line following incubation with TGF-β1 at various concentrations for 20 h was examined by Flow cytometry. Data were expressed as mean ± SD and were representative of 5 separate experiments performed in quadruplicate, **p < 0.01.
