Figure 3

TGF-β1 increased TREM-1 expression in a time- and dose-dependent manner in mouse primary alveolar macrophages.
(a,b) TREM-1 protein level on the surface of mouse primary alveolar macrophages after stimulation with 10 ng/mL TGF-β1 for various periods of time (0, 1, 2, 5, 10, 20 and 40 h) was tested by Flow cytometry. (c,d) The protein content of TREM-1 of macrophages following incubation with TGF-β1 at various concentrations (0, 1, 2, 5, 10 and 20 ng/mL) for 20 h was examined by Flow cytometry. Data were expressed as mean ± SD and were representative of 2 separate experiments performed in triplicate, **p < 0.01.