Figure 4
The TGF-β1 mediated increase in TREM-1 expression might be associated with AP-1 in mouse macrophages.
Expression of AP-1 mRNA in lung tissues (a) was determined by real-time PCR. Correlations between AP-1 and TGF-β1 (b), between AP-1 and TREM-1 (c) by Pearson correlation analysis, the correlation coefficients were 0.773 and 0.773 (p < 0.01), respectively. The expression of AP-1 mRNA in 10 ng/ml TGF-β1 stimulated mouse macrophages at different time points (0, 1, 2, 5, 10, 20 and 40 h) and different concentrations for 20 h (0, 1, 2, 5, 10 and 20 ng/mL) (d,e) was detected by real-time PCR. Data were expressed as mean ± SD and were representative of 5 separate experiments performed in 8 mice or quadruplicate per group, **p < 0.01.
