Figure 5
TGF-β1 stimulation increased the transcriptional activity of AP-1 at the TREM-1 promoter.
Mouse macrophages were transfected with pGL3-basic vector (500 ng), or WT-TREM1 reporter plasmid (500 ng), or mutant-TREM1 reporter plasmid (500 ng) and the renilla luciferase expressing pRL-TK plasmid (1 ng, an internal control) and were incubated for 5 h. Cells were then incubated in the absence or presence of TGF-β1 (10 ng/mL) for 20 h. Firefly and renilla luciferase activities were detected using Varioskan Flash. The relative luciferase activities were standardized to renilla luciferase activity. Data were expressed as mean ± SD and were representative of 5 separate experiments performed in quadruplicate, **p < 0.01.
