Table 3 Microsatellite markers development and their application in a tested population.

From: Distribution, function and evolution characterization of microsatellite in Sargassum thunbergii (Fucales, Phaeophyta) transcriptome and their application in marker development

Loci

Primer sequences (5′-3′)

Repeat motif

Ta (°C)

Size range (bp)

NA

PIC

HO

HE

SW1

F:AACGGAAGCGCAATACGAC

(AC)11

60

411–421

5

0.653

0.633

0.720

 

GCAGACACGGTTGACGAAG

       

SW6

CAAAGTTGCTGCGTGATTCG

(CT)11

60

160–164

2

0.375

0.433

0.508

 

CACGATGTGTCGCCTTCTG

       

SW9

AAAGTTGCTGAGCCGTTCG

(ACC)8

60

435–456

4

0.694

0.433

0.754

 

CAGGAGGACCATCGATCCC

       

SW10

TGGCTGTGTGGATACGACC

(ACT)8

60

332–353

5

0.651

0.566

0.714

 

TGTCGCAATGCTCGTTGTAG

       

SW16

CCCAAATCAGCGAAAGGCG

(GTT)21

61

402–411

3

0.342

0.333

0.422

 

CGGTGCTACGATACTGCCC

       

SW17

GCCTTCGTTACGCTTGACC

(ATTT)6

60

364–372

5

0.420

0.100

0.473

 

TACCACCTGAGCAATCCCG

       

SW18

ACCCGACGAGCTCTACAAG

(CAGT)6

60

428–452

5

0.427

0.333

0.465

 

TGAGTGGGTTGAAGACGGG

       

SW21

ATGCCAGGAGCTACACAGG

(AAACC)7

60

383–400

4

0.418

0.600

0.532

 

AGATGGCTCAACCTCTGCC

       

SW24

TTGCCCGGGTATCCTGTTC

(AAACAT)4

60

310–316

2

0.339

0.300

0.440

 

TTTCGCGTTGAGCACTTCG

       

SW35

GCTATGTCAACAACCACCTCT

(ATC)4…(ATC)4

59

332–348

4

0.411

0.500

0.521

 

TTCTGATTCGAGGTATTGTGC

       
  1. Ta: annealing temperature; NA: The observed number of alleles; PIC: Polymorphism Information Content; Ho: the mean observed heterozygosity; HE: the mean expected heterozygosity.
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