Figure 2
Pharmacological inhibition of autophagy by CQ increases sensitivity of BRAFV600E CRC cells to PLX4032.
(A) HT29 cells were treated with or without 0.1, 1, or 10 μM PLX4032 in the presence or absence of 0, 5, 25, or 50 μM CQ for 24 h. Cell viability was determined using MTT assays. (B) Isobologram analysis of interactions between PLX4032 and CQ in HT29 cells. (C) RKO cells were treated with or without 0.1, 1, or 10 μM PLX4032 in the presence or absence of 0, 5, 25, or 50 μM CQ for 24 h. Cell viability was determined using MTT assays. (D) Isobologram analysis of interactions between PLX4032 and CQ in RKO cells. (E) HT29 and RKO cells were treated with PLX4032 or PLX4720 at 10 μM in the presence or absence of CQ at 25 μM for 24 h. Apoptosis rates were determined using flow cytometry and cells were defined as early or late apoptotic cells. Data are presented as means and standard deviations of at least three independent experiments; *p < 0.05.
