Figure 3
Inhibition of autophagy by Atg13 or Atg7 siRNA increases sensitivity of BRAFV600E CRC cells to PLX4032.
(A,B) HT29 cells were transfected with Atg13 siRNA or control siRNA and were then treated with DMSO and PLX4032 or PLX4720 at 10 μM for 24 h. LC3 and Atg13 expression was determined using western blot analyses. (C,D) HT29 and RKO cells were transfected with Atg7 siRNA or control siRNA and were then treated with DMSO or PLX4032 at 10 μM for 24 h. LC3 and Atg7 expression was determined using western blot analyses. (E,F) HT29 and RKO cells were transfected with control siRNA, Atg13 siRNA, or Atg7 siRNA and were treated with DMSO, PLX4032, or PLX4720 at 10 μM for 24 h. Cell viability was then determined using MTT assays. (G,H) Apoptosis assay in HT29 and RKO cells transfected with control siRNA, Atg13 siRNA, or Atg7 siRNA. Apoptotic rates were determined using flow cytometry and early and late apoptotic cells were distinguished. Data are presented as means and standard deviations of at least three independent experiments; *p < 0.05.
