Figure 4 | Scientific Reports

Figure 4

From: BRAFV600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells

Figure 4

AMPK phosphorylation is required for ULK1 function during PLX4032-induced autophagy.

(A) Western blot assays of AMPKα (Thr172) and mTOR (Ser2448) phosphorylation and LC3 expression in HT29 and RKO cells after treatment with DMSO for 24 h and 10 μM PLX4032 for 2, 6, 12, or 24 h. (B) Western blot analyses of phospho-AMPKα (Thr172), phospho-ULK1 (Ser555), phospho-Raptor (Ser792), phospho-ULK1 (Ser757) and LC3 in HT29 cells after treatment with DMSO and 10 μM PLX4032 for 24 h. (C) Western blot analyses of phospho-AMPKα (Thr172), phospho-ULK1 (Ser555) and LC3 in HT29 cells after treatment with DMSO for 24 h and 10 μM PLX4032 for 2, 6, 12 and 24 h. (D) Western blot analyses of phospho-AMPKα (Thr172), phospho-ULK1 (Ser555), phospho-Raptor (Ser792), phospho-ULK1 (Ser757) and LC3 in RKO cells after treatment with DMSO and 10 μM PLX4032 for 24 h. (E) Western blot analyses of phospho-AMPKα (Thr172), phospho-ULK1 (Ser555), phospho-Raptor (Ser792), phospho-ULK1 (Ser757) and LC3 in RKO cells after treatment with DMSO for 24h and 10 μM PLX4032 for 2, 6, 12 and 24 h.

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