Figure 6
From: Molecular mapping and characterization of the silkworm apodal mutant
Expression profiles of Hox genes in Dazao and the ap mutant and the interaction between the Bmsob protein and Hox genes.
(a) Expression profiles of Hox genes in Dazao and the ap mutant. **P < 0.01, Student’s t-test, n = 3. Detection by EMSA of (b) the interaction between the Bmsob protein and the BmAntp gene; (c) the interaction between the Bmsob protein and the BmUbx gene; (d) the interaction between the Bmsob protein and the BmAbd-B gene. The amount of GST protein used in the reactions was 1 μg and the amount of Bmsob protein was 0.8 μg. The amount of biotin labeling probe, in turn, was 1, 2 and 6 pmol. The amount of unlabeled probe, in turn, was 2, 20 and 100 pmol. The arrow indicates the protein-DNA complex.
