Figure 7
From: Molecular mapping and characterization of the silkworm apodal mutant
Expression profiles of the BmDsp gene in Dazao and the ap mutant, the interaction between the Bmsob protein and the BmDsp gene and the interaction between the BmDsp protein and the Hox genes.
(a) Expression profiles of BmDsp genes in Dazao and the ap mutant. **P < 0.01, Student’s t-test, n = 3. Detection by EMSA of (b) the interaction between the Bmsob protein and the BmDsp gene; (c) the interaction between the BmDsp protein and the BmAntp gene; (d) the interaction between the BmDsp protein and the BmUbx gene; (e) the interaction between the BmDsp protein and the BmAbd-B gene. The amount of protein used in each reaction was: GST 1 μg; Bmsob 0.8 μg; BmDsp 0.8 μg. The amount of biotin labeling probe, in turn, was 1, 2 and 6 pmol. The amount of unlabeled probe, in turn, was 2, 20 and 100 pmol. The arrow indicates the protein-DNA complex. (f) Predicted regulation relationship between the Bmsob, BmDsp and Hox genes.
