Figure 5
From: Resolvin D4 stereoassignment and its novel actions in host protection and bacterial clearance
RvD4 reduces neutrophil infiltration in zymosan-initiated peritonitis and S. aureus infection.
6–8-week-old male FVB mice were injected i.p. with 10 ng RvD3, RvD4, or vehicle (saline containing 0.01% EtOH) along with 1 mg zymosan A. Exudates were collected 4 hr later. (A) Total cell counts were enumerated using light microscopy and PMN were identified using flow cytometry. Results are mean ± SEM, n = 3–4 mice. Statistical analysis was performed using two-way ANOVA. *p < 0.05, **p < 0.01, vs. zymosan A alone. Murine dorsal pouches were inoculated with live S. aureus (105 c.f.u.) followed by injection of vehicle or RvD4 (200 ng/mouse); exudates were collected at 4, 12, 24 and 48 hr; (B) Exudate PMN numbers and resolution indices were calculated; (C) H & E staining of airpouch lining (arrows denote PMN; (D) Bacterial counts were expressed as colony forming unit (c.f.u.); (E–G) Endogenous eicosanoids in infectious exudates from mice given vehicle or RvD4 (200ng/mouse); (E) MRM chromatogram for exudate eicosanoids; (F) Representative MS-MS spectra employed for the identification of PGD2; (G) Exudate prostanoid levels. Results are mean ± SEM, n = 3 separate experiments. Statistical analysis was performed using Student’s t-test. *p < 0.05, vs. S. aureus alone.
