Table 2 Phylum-level assignments of operational taxonomic units (OTUs) in the musk pods of unmated and mated Chinese forest musk deer males.

From: The musk chemical composition and microbiota of Chinese forest musk deer males

Class

Phylum

Number of OTUs

Percentage out of total number of OTUs (%)

Number of reads

Percentage out of total number of reads (%)

MM

UM

MM

UM

MM

UM

MM

UM

Bacteria

Proteobacteria

351.6 ± 40.7 a

133.2 ± 8.9 b

32.73 a

23.19 b

1948.6 ± 223.6 a

774.6 ± 137.3 b

29.44 a

11.88 b

Ā 

Bacteroidetes

74.6 ± 9.7

52.2 ± 6.4

6.94

9.12

118.6 ± 20.8

330.8 ± 84.7

1.72 a

4.92 b

Ā 

Firmicutes

86.2 ± 5.4 a

167 ± 13.8 b

8.26 a

29.11 b

359.0 ± 39.3 a

2112.6 ± 388.4 b

5.45 a

32.32 b

Ā 

Actinobacteria

52.2 ± 9.6

58.2 ± 3.2

4.72 a

10.14 b

362.4 ± 38.6 a

1720.0 ± 280.2 b

5.50 a

26.63 b

Ā 

Fusobacteria

6.2 ± 0.7

5.4 ± 0.4

0.62

0.94

34.2 ± 9.1 a

996.6 ± 228.7 b

0.47 a

14.65 b

Ā 

OD1

3.6 ± 1.3

1.0 ± 0.3

0.4

0.17

4.4 ± 1.8

2.2 ± 1.0

0.08

0.03

Ā 

WS3

1 ± 0.5

0.2 ± 0.2

0.08

0.03

1.4 ± 0.9

0.2 ± 0.2

0.02

0.00

Ā 

Cyanobacteria

5.2 ± 0.7

6.8 ± 0.9

0.50a

1.18b

10.2 ± 2.2

10.6 ± 1.4

0.14

0.18

Ā 

Verrucomicrobia

8 ± 2.2

2.8 ± 0.6

0.7

0.48

16.2 ± 4.2

5.8 ± 1.3

0.24

0.11

Ā 

Tenericutes

3.6 ± 0.5

2.8 ± 0.5

0.35

0.49

55.2 ± 9.9

18.6 ± 6.5

0.85

0.36

Ā 

Synergistetes

1.4 ± 0.2

1.2 ± 0.5

0.14

0.22

1.6 ± 0.4

1.6 ± 0.7

0.02

0.02

Ā 

Spirochaetes

0.4 ± 0.2

0.2 ± 0.2

0.04

0.03

0.6 ± 0.4

0.2 ± 0.2

0.01

0.00

Ā 

TM7

8.6 ± 1.5a

2.6 ± 0.7b

0.77

0.44

15.2 ± 3.8

3.6 ± 1.3

0.21a

0.06b

Ā 

Planctomycetes

13.8 ± 2a

4.6 ± 0.4b

1.29a

0.81b

19 ± 2.7a

5.4 ± 0.8b

0.28a

0.10b

Ā 

Nitrospira

3.2 ± 1.2

1.4 ± 0.4

0.24

0.25

5.6 ± 1.9

1.4 ± 0.4

0.08

0.03

Ā 

Gemmatimonadetes

20.2 ± 3.3

9.8 ± 2.1

1.84

1.73

29.4 ± 5.9

11.2 ± 2.3

0.42

0.22

Ā 

Fibrobacteres

0.4 ± 0.2

0.0 ± 0.0

0.04

0

0.6 ± 0.4

0.0 ± 0.0

0.01

0.00

Ā 

Elusimicrobia

1.4 ± 0.7

0.0 ± 0.0

0.11

0

1.6 ± 0.8

0.0 ± 0.0

0.02

0.00

Ā 

Deinococcus-Thermus

1 ± 0.3

1.4 ± 0.5

0.09

0.25

1.6 ± 0.5

1.8 ± 0.7

0.02

0.03

Ā 

Deferribacteres

0.2 ± 0.2

0.0 ± 0.0

0.01

0

0.2 ± 0.2

0.0 ± 0.0

0.00

0.00

Ā 

Chrysiogenetes

0.4 ± 0.2

0.2 ± 0.2

0.03

0.03

0.8 ± 0.6

0.2 ± 0.2

0.01

0.00

Ā 

Chloroflexi

10.8 ± 2.1a

2.6 ± 0.2b

0.98

0.45

14.0 ± 3.4

2.6 ± 0.2

0.20a

0.05b

Ā 

Chlorobi

2 ± 0.6

0.4 ± 0.2

0.17

0.07

10.2 ± 1.4a

0.6 ± 0.4b

0.16a

0.01b

Ā 

Chlamydiae

2.4 ± 0.5

0.6 ± 0.2

0.24

0.1

3.6 ± 0.6

1 ± 0.6

0.06

0.02

Ā 

Caldiserica

0.8 ± 0.2a

0.0 ± 0.0 b

0.07a

0.00b

0.8 ± 0.2a

0.0 ± 0.0b

0.01a

0.00b

Ā 

Armatimonadetes

0.4 ± 0.2

0.6 ± 0.2

0.03

0.11

0.4 ± 0.2

0.6 ± 0.2

0.00

0.01

Ā 

Acidobacteria

54.8 ± 14.3

16.8 ± 2.8

4.73

2.95

73.2 ± 20.6

21.4 ± 2.9

1.00

0.39

Ā 

Bacteria unknown

39.2 ± 7.1a

11.4 ± 1.6b

3.53a

1.98b

46.6 ± 8.2a

12.8 ± 1.8b

0.68a

0.23b

Archaea

Euryarchaeota

318.8 ± 35.3 a

88.2 ± 20.1 b

29.81 a

15.19 b

3779.2 ± 850.7 a

414.4 ± 120.5 b

52.67 a

7.67 b

Ā 

Crenarchaeota

3.6 ± 0.4

2.4 ± 0.2

0.36

0.41

14.4 ± 5.6

4.2 ± 1.2

0.19

0.07

Ā 

Archaea unknown

1.6 ± 0.4

0.4 ± 0.2

0.15

0.06

2.0 ± 0.6

1.0 ± 0.6

0.03

0.01

Unknown

Unknown

0.6 ± 0.4

0.2 ± 0.2

0.03

0.07

0.8 ± 0.6

0.2 ± 0.2

0.01

0.00

  1. Note: After Bonferroni correction, reads number and OTU number are represented as least squares means ± standard deviation. Associations between mating status and phylum-level assignments were assessed using the least-squares method (GLM procedure, SAS version 8.02, 2001). Significance of the least squares means was tested with the Tukey’s Multiple Range test. Least squares means in a line with different superscripts are significantly (a,bP < 0.01) different. Percentage of OTU number and percentage of reads number are represented as median values. Median percentage values of bacterial and archaea phyla in samples from mated and unmated males found by using the RDP Classifier. Significance of the median percentage values was tested with the Mann-Whitney U test (a,bP < 0.01). More than 95% of the sequences in all samples were found to belong to the six most populated underlined phyla.