Figure 1
Behavioral responses to neuropathic pain in female and male mice (A) Mechanical nociceptive threshold.
Sex-related differences in the development of mechanical allodynia induced by Chronic Constriction Injury (CCI): time course of withdrawal thresholds (expressed as applied force in grams) of hindpaws ipsi- and contralateral to the injury in OIL (ipsi 
, contra ○) and 17β-estradiol (17β-E) (ipsi ▲, contra △), female (left panels) and male (right panels) mice. Two-way ANOVAs for repeated measures showed significant differences for treatment (F1,18 = 101.268, P < 0.0001 and F1,18 = 106.527, P < 0.0001, for females and males respectively), time (F19,342 = 25.273, P < 0.0001 and F19,342 = 56.858, P < 0.0001, for females and males respectively) and for treatment x time interaction (F19,342 = 2.835, P < 0.0001 and F19,342 = 3.764, P < 0.0001, for females and males respectively). Number of mice was 10 for each experimental group. (*)P < 0.05; (* *)P < 0.01; (***)P < 0.001 vs OIL. (B) Weight bearing. Weight percent distribution time course of the hind limb ipsilateral to the injury in OIL () and 17β-estradiol (17β-E) (▲), female (left panels) and male (right panels) mice. Two-way ANOVAs for repeated measures showed significant differences for treatment (F1,18 = 17.823, P < 0.0005 and F1,18 = 20.498, P < 0.0005 for females and males respectively), time (F19,342 = 62.9641, P < 0.0001 and F19,342 = 70.249, P < 0.0001 for females and males respectively) and treatment x time interaction (F19,342 = 2.932, P < 0.0001 and F19,342 = 11.045, P < 0.0001 for females and males respectively). Number of mice was 10 for each experimental group. (*)P < 0.05; (* *)P < 0.01; (***)P < 0.001 vs OIL.
