Figure 2 | Scientific Reports

Figure 2

From: 17beta-estradiol counteracts neuropathic pain: a behavioural, immunohistochemical and proteomic investigation on sex-related differences in mice

Figure 2

Sex-dependent differences in the expression and quantification of ERα, S100β and nuclei in sciatic nerves at CCI D7.

(A,B) Representative examples of high magnification (63X) IF images of S100β (green), ERα (red) and colocalization between S100β and ERα (merge, yellow), taken from sciatic nerves of OIL or 17β-estradiol female (A) and male (B) mice. Scale bar: 50 μm. White arrows indicate areas shown in the inset squares (63x, digital zoom 3). Right panels show quantification of S100β and ERα in OIL and 17β-estradiol (17β-E) mice by using RGB method that converts pixel in brightness values. (*)P < 0.05 vs OIL. (C) Histograms show the total number of nuclei (Hoechst positive cells) in OIL and 17β-estradiol female and male mice. (*)P < 0.05 vs OIL.

Back to article page