Figure 3 | Scientific Reports

Figure 3

From: 17beta-estradiol counteracts neuropathic pain: a behavioural, immunohistochemical and proteomic investigation on sex-related differences in mice

Figure 3

Sex-dependent differences in the expression and quantification of Neu200 and P0 in sciatic nerves at CCI D7.

(A,B) Representative examples of high magnification (63X) IF images of Neu200 (green), P0 (red) and colocalization between Neu200 and P0 (merge, yellow), taken from sciatic nerves of OIL and 17β-estradiol female (A) and male (B) mice. Scale bar: 50 μm. White arrows indicate areas shown in the inset squares (63x, digital zoom 3). Right panels show quantification of Neu200 and P0 in OIL and 17β-estradiol (17β-E) mice by using RGB method that converts pixel in brightness values. (*)P < 0.05 vs OIL. (C,D) Representative examples of Western blot analysis of Neu200 and P0 protein expression in sciatic nerves from OIL or 17β-estradiol (17β-E) female (C) and male (D) mice. Histograms show quantification of Neu200 (green) and P0 (red) as obtained using the software ImageJ software (version 1.41, National Institutes of Health, USA).

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