Figure 4

Sex-dependent differences in the expression and quantification of Neu200 and P0 in sciatic nerves at CCI D121.
(A,B) Representative examples of high magnification (63X) IF images of Neu200 (green), P0 (red) and colocalization between Neu200 and P0 (merge; yellow), taken from sciatic nerves of OIL and 17β-estradiol female (A) and male (B) mice. Scale bar: 50 μm. White arrows indicate areas shown in the inset squares (63x, digital zoom 3). Right panels show quantification of Neu200 and P0 in OIL and 17β-estradiol mice by using RGB method that converted pixel in brightness values.