Figure 5

Sex-dependent differences in the expression/activation of spinal astrocytes and microglia, at CCI D7 and CCI D121.
Representative examples of low magnification (10X) IF images of GFAP (astrocytes; green) and CD11b (microglia; green) in L4/L5 spinal cord sections taken from OIL and 17β-estradiol female and male mice at CCI D7 (A) and at CCI D121 (B). Scale bar: 300 μm. White squares indicate the ipsilateral dorsal horn considered for acquisition of the representative examples of high magnification (63X) IF images reported in Figs 6, 7, 8.