Figure 6
Sex-dependent differences in the expression/activation of spinal astrocytes at CCI D7.
(A,B) Representative examples of high magnification (63X) IF images of GFAP (astrocytes; green), p-p38 (phosphorylated-p38, red) and their colocalization (merge; yellow) in L4/L5 spinal cord ipsilateral sections taken from OIL and 17β-estradiol female (A) and male (B) mice at CCI D7. Scale bar: 50 μm. Histograms show quantification of total number of GFAP-IR cells (green) and their colocalization with p-p38 (yellow) in OIL and 17β-estradiol (17β-E) female and male mice. Full columns indicate the side of spinal cord ipsilateral to the ligature, hatched columns indicate the contralateral side. (*)P < 0.05 vs OIL GFAP-IR. (C,D) Representative examples of high magnification (63X) IF images of GFAP (astrocytes; green), ERα (anti-Estrogen-related Receptor α; red) and their colocalization (merge: yellow) in L4/L5 spinal cord ipsilateral sections taken from OIL and 17β-estradiol female (C) and male (D) mice at CCI D7. Histograms show quantification of total number of GFAP-IR cells (green) and their colocalization with ERα (yellow) in OIL and 17β-estradiol (17β-E) female and male mice. Full columns indicate the side of spinal cord ipsilateral to the ligature, hatched columns indicate the contralateral side. (*)P < 0.05 vs OIL GFAP-IR; (°) P < 0.05 vs OIL GFAP-ERα-IR.
