Figure 7
Sex-dependent differences in the expression/activation of spinal microglia at CCI D7.
(A,B) Representative examples of high magnification (63X) IF images of CD11b (microglia; green), p-p38 (phosphorylated-p38; red) and their colocalization (merge, yellow) in L4/L5 spinal cord ipsilateral sections taken from OIL and 17β-estradiol female (A) and male (B) mice at CCI D7. Scale bar: 50 μm. Histograms show quantification of total number of CD11b-IR cells (green) and their colocalization with pp38 (yellow) in OIL and 17β-estradiol (17β-E) female and male mice. Full columns indicate the side of spinal cord ipsilateral to the ligature, hatched columns indicate the contralateral side. (***)P < 0.001 vs OIL CD11b-IR; (°°°) P < 0.001 vs OIL CD11b-pp38-IR. (C,D) Representative examples of high magnification (63X) IF images of CD11b (microglia; green), ERα (anti-Estrogen-related Receptor α; red) and their colocalization (merge; yellow) in L4/L5 spinal cord ipsilateral sections taken from OIL and 17β-estradiol female (C) and male (D) mice at CCI D7. Scale bar: 50 μm. Histograms show quantification of total number of CD11b-IR cells (green) and their colocalization with ERα (yellow) in OIL and 17β-estradiol (17β-E) female and male mice. Full columns indicate the side of spinal cord ipsilateral to the ligature, hatched columns indicate the contralateral side. (***)P < 0.001 vs OIL CD11b-IR; (°) P < 0.05, (°°°) P < 0.001 vs OIL CD11b- ERα-IR.
