Figure 8
Sex-dependent differences in the expression/activation of spinal astrocytes and spinal microglia at CCI D121.
(A,B) Representative examples of high magnification (63X) IF images of GFAP (astrocytes; green), p-p38 (phosphorylated-p38; red) and their colocalization (merge, yellow) in L4/L5 spinal cord ipsilateral sections taken from OIL and 17β-estradiol female (A) and male (B) mice at CCI D121. Scale bar: 50 μm. Histograms show the quantification of total number of GFAP IR cells (green) and their colocalization with pp38 (yellow) in OIL and 17β-estradiol (17β-E) female and male mice. Full columns indicate the side of spinal cord ipsilateral to the ligature, hatched columns indicate the contralateral side. (*)P < 0.05 vs OIL GFAP-IR; (°°) P < 0.01 vs OIL GFAP-pp38-IR. (C,D) Representative examples of high magnification (63X) IF images of CD11b (microglia; green), p-p38 (phosphorylated-p38; red) and their colocalization (merge, yellow) in L4/L5 spinal cord ipsilateral sections taken from OIL and 17β-estradiol female (C) and male (D) mice at CCI D121. Scale bar: 50 μm. Histograms show the quantification of total number of CD11b-IR cells (green) and their colocalization with pp38 (yellow) in OIL and 17β-estradiol (17β-E) female and male mice. Full columns indicate the side of spinal cord ipsilateral to the ligature, hatched columns indicate the contralateral side. (**)P < 0.01 vs OIL CD11b-IR; (°) P<0.05 vs OIL CD11b-pp-38-IR.
