Figure 3
Arabidopsis plants expressing PPOA have reduced growth rate and delayed bolting time.
(a) RT-qPCR analysis of PPO mRNA abundance in PPOM and PPOA lines. Data are represented relative to PPOM line 7 and correspond to mean ± SEM derived from three technical repeats of three biological replicates. Data correspond to 4-week-old plants. (b) PPO activity for PPOM and PPOA lines. Data are represented relative to PPOM line 7 and correspond to mean ± SEM derived from three technical repeats of three biological replicates. Data correspond to 4-week-old plants. (c) Phenotype of PPOM and PPOA lines. Data correspond to 4-week-old plants. (d) Surface area of rosette leaves for PPOM and PPOA lines. Data correspond to mean ± SEM derived from 10 biological replicates. Asterisks represent statistically significant differences (***P = 0.001) according to the ANOVA followed by Newman-Keuls post-hoc test. Data correspond to 4-week-old plants. (e) Bolting time in PPOM and PPOA lines. Data correspond to mean ± SEM derived from 10 biological replicates. Asterisks represent statistically significant differences (*P = 0.05, ***P = 0.001) according to the ANOVA followed by Newman-Keuls post-hoc test.
