Figure 4
Qualitative and quantitative profiles of phenylpropanoid metabolites in transgenic and untransformed Arabidopsis seedlings.
Transgenic PPOM and PPOA lines together with untransformed (WT) controls were grown on sucrose-supplemented medium and used for phenylpropanoid profiling as described in Materials and methods. (a) Representative HPLC chromatograms of flavonols. (b) Representative HPLC chromatograms of anthocyanins. (c) Total flavonol and anthocyanin levels represented relative to WT samples. Data correspond to mean ± SEM derived from three technical repeats of four biological replicates. Asterisks mark statistically significant differences (***P = 0.001) according to the ANOVA followed by Newman-Keuls post-hoc test. (d) Phenotype of seedlings grown for 10 days in the presence of norflurazon. Note the stronger anthocyanin (purple) pigmentation in PPOA seedlings. Scale bar represents 0.5 cm.
