Figure 3

Effects of addition of the mixed sugars on glycerol metabolism and 1,3-PD production by C. diolis DSM 15410.
Batch fermentation was carried out at 34 °C in a 5-L bioreactor and the pH was maintained at 6.8 by automatic addition of 2 M KOH. The culture was stirred at 100 rpm and sparged with nitrogen at a flow rate of 0.1 volume of gas per volume of liquid per minute. The initial concentration of glycerol is approximately 20.0 g/L. Mixed sugars (glucose:xylose:arabinose = 1:1:1) were added as co-substrates. The error bars indicate the standard deviation among the three parallel replicates.