Figure 4 | Scientific Reports

Figure 4

From: Co-utilization of glycerol and lignocellulosic hydrolysates enhances anaerobic 1,3-propanediol production by Clostridium diolis

Figure 4

Effects of the mixed sugars on transcriptional levels of key genes involved in glycerol metabolism and internal redox reducing equivalent in C. diolis DSM 15410.

(a) Determination of relative transcriptional levels of dhaB1, dhaT and dhaD using quantitative PCR. (b) Determination of intracellular NADH/NAD+ ratio. Cells were cultured using glycerol and mixed sugars (glucose:xylose:arabinose = 1:1:1) as co-carbon sources. Samples were collected in the middle of the exponential phase when OD620 was approximately 1.0. At the same time, cells cultured in glycerol medium were taken as control. The initial glycerol concentration was 20.0 g/L and the total sugars concentration was 10.0 g/L. The error bars indicate the standard deviation among the three parallel replicates.

Back to article page