Figure 3
From: Length of intact plasma membrane determines the diffusion properties of cellular water
Molecular diffusion of camptothecin-treated (apoptotic) HeLa S3 cells.
(a) Confocal microscopy for DAPI staining (upper panels) showing the fragmented nuclei of camptothecin-treated (24 h) HeLa S3 cells. Differential interference microscopy (lower panels) showing blebbing (arrows). Scale bar = 10 μm. (b) Graph showing decreases in cell volume of camptothecin-treated HeLa S3 cells. * Significantly different from values at 0 h (p < 0.001; t-test). (c) Phase-contrast micrographs (upper panels) show non-treated (0 h) or 1 μM camptothecin-treated (8 h and 24 h) HeLa S3 cells. Cells are rounded and detach from the dish bottoms as early as 8 h after the addition of camptothecin in culture medium. FACS analysis (lower panels) show time-dependent increases in annexin-positive cell fraction of camptothecin-treated cells. Bars with percentages in graphs indicate annexin-positive (apoptotic) cell fractions. (d) Transmission electron microscopy showing chromatin condensation and blebbing of the intact plasma membrane of camptothecin-treated (24 h) HeLa S3 cell. Scale bar = 2 μm. (e) Schematic representation and cellular characteristics of cell pellets containing camptothecin-treated (24 h, apoptotic) HeLa cells. (f) Graph showing the time-dependent decreases in ADC values of camptothecin-treated (8 h and 24 h) HeLa S3 cells. All cells that adhered to or detached from the bottom of culture dishes were collected, resuspended in PBS, centrifuged and assessed for ADC values. The data are expressed as the mean ± s.d. from 3 independent experiments. * Significantly different from the ADC values at 0 h (p < 0.001; Tukey-Kramer test).
