Figure 4

Molecular diffusion of TNFα-treated (necroptotic) L929 cells.

(a) Confocal microscopy for DAPI staining (upper panels) and differential interference microscopy (lower panels) showing intact nuclei of TNFα-treated (48 h) L929 cells. Scale bar = 10 μm. (b) Graph showing early increases (8 h) and subsequent decreases (48 h) in the cell volume of TNFα-treated L929 cells. *^, ** Significantly different from the cell volumes at 0 h (*p < 0.001, **p < 0.01; Tukey-Kramer test). (c) Graph showing early increases (3 h and 8 h) and subsequent decreases (24 h and 48 h) in the cell viability of TNFα-treated L929 cells. *^, **^, *** Significantly different from cell viability at 0 h (* p < 0.05, **p < 0.01, ***p < 0.001; Tukey-Kramer test). (d) Transmission electron microscopy showing disruption of the plasma membrane and swelling of the mitochondria of TNFα-treated (48 h) L929 cells. Scale bar = 2 μm. (e) Schematic representation and cellular characteristics of cell pellets containing TNFα-treated (necroptotic, 8 h and 48 h) L929 cells. (f) Graph showing a gradual increase in the ADC value of necroptotic L929 cells.* Significantly different from the ADC values at 0 h (p < 0.001; Tukey-Kramer test).