Figure 3
From: Biophysical regulation of Chlamydia pneumoniae-infected monocyte recruitment to atherosclerotic foci
C. pneumoniae infection increases membrane fluidity and uniformity of lipid raft distribution.
Uninfected (mock) or infected (Cpn) monocytes were analyzed for fluidity of membrane using a fluorescence plate reader and the results were plotted as ratio of emissions at 480 and 400 nm (A); analyzed for total cholesterol using fluorescence plate reader (B); stained for membrane lipid rafts (green), actin (red) and nucleus (blue) and visualized by confocal microscopy (Scale bar, 10 μm) (C). The results are mean ± SEM of five different experiments performed in triplicate. The statistical significance in the parameters between the groups was shown as p value from t test. In another experiment monocytes were stained for lipid rafts and analyzed for lipid raft distribution by spot count analysis (D); and homogeneity (E) by imaging flow cytometry. The results are from one representative experiment performed in triplicate and all the experiments were repeated five times.
