Figure 1 | Scientific Reports

Figure 1

From: Dihydroartemisinin prevents breast cancer-induced osteolysis via inhibiting both breast caner cells and osteoclasts

Figure 1

DHA inhibited RANKL-induced osteoclastogenesis and gene expression.

(A) BMMs were treated with various concentrations of DHA, 30 ng/mL M-CSF and 50 ng/mL RANKL for 5–7 days and then subjected to TRAP staining. (B) The numbers and areas of osteoclasts from panel A were measured. (C) BMMs were treated with or without 1.56 μM DHA followed by 30 ng/mL M-CSF and 50 ng/mL RANKL for 3, 5 and 7 days and BMMs were subjected to TRAP staining. (D) The numbers and areas of osteoclasts from panel C were measured. (E) BMMs were cultured until mature osteoclasts were observed with different concentrations of DHA, 30 ng/mL M-CSF and 50 ng/mL RANKL and then the osteoclast-specific gene expression was measured using real-time PCR. (*p < 0.05, **p < 0.01).

Back to article page