Figure 5
From: Targeted pruning of a neuron’s dendritic tree via femtosecond laser dendrotomy
Changes in temporal firing pattern in response to current injection.
Experimentally acquired f-I plots before (solid circles) and after (hollow circles) dendrotomy of representative (a) 4th and (b) 1st order dendrites, 600 μm and 360 μm from the soma, respectively. Solid lines denote exponential fit. The insets show representative firing patterns for Istep = 400 pA. Scale bars 80 mV, 100 ms. (c) Mean differences between pre- and post-dendrotomy firing frequencies acquired via experiment for different current step amplitudes plotted against the ratio of the value of Rin after dendrotomy to its initial value. **denotes P < 0.001 for comparison of corresponding f-I curves before and after dendrotomy. (d–f) Simulation plots similar to (a–c) Experimental data points in c correspond to different cells while in d, all numerically acquired data points are from a single model cell. The dendrotomy sites are shown in Fig. 4a.
