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Figure 1

From: Enhanced engraftment, proliferation and therapeutic potential in heart using optimized human iPSC-derived cardiomyocytes

Figure 1

Cardiac differentiation and characteristics of iPSC-derived cardiomyocytes.

(a) Schematic representation of the cardiac differentiation protocol. (b) Representative images of differentiated embryoid bodies. Left: bright-field; right: GFP. Scale bars: 100 μm. (c) Increasing number of cells per embryoid body during cardiac differentiation (n = 3). (d) Percentage of GFP-positive cells during differentiation (n = 3).(e) Immunostaining on day 20 of purified CMs: red, cTNT; blue, Hoechst. Scale bar: 20 μm. (f) Percentage of Ki67-positive CMs in vitro (n = 3) over different days. Data are represented as mean ± SD. (g) Hierarchical clustering of the global gene expression data obtained from undifferentiated iPSCs, day4 mesodermal cells, adult and fetal human hearts and purified day8, 20, 30 and 80 iPSC-CMs. (h) Gene ontology analysis of differentially expressed genes between day20 iPSC-CMs and adult heart (fold change > 2.0, p < 0.05).

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