Figure 7
Optimized cell transplantation using another iPSC line purified by cell sorting for the cardiac surface marker, SIRPA.
(a) Flow cytometry analysis 20 days after initial differentiation. SIPRA-positive and CD31, 49a, 90, 140b and TRA1-60-negative cells were sorted. (b) Flow cytometry analysis of the sorted cells stained by cTNT. (c) Follow-up bioluminescence imaging of injected day20 purified-CMs. Values are mean ± SE. (d) Immunostaining of engrafted CMs 6 months after the initial injection of day20 CMs for cTNT (red), anti-luc (green) and Hoechst (blue). Scale bars: 300 μm. (e) Immunostaining of engrafted CMs at high magnification: red, actinin; yellow, anti-luc; blue, Hoechst. Scale bars: 20 μm.
