Figure 3
From: REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering
Modifications in larger plasmids.
The 24 kb Wnt1 targeting vector (undigested; lane 1) contains three MfeI restriction sites, which upon MfeI digestion gives three bands (15476, 6379, 1844 bp; lane 2). One of the MfeI sites is mutated by REPLACR-mutagenesis, such that the resulting plasmid (lane 3) upon MfeI digestion gives only two bands (15476 and 8225 bp; lane 4).
