Figure 4
ASI decreased microglia activation depending on GR.
(A) ASI inhibited NO production in BV-2 cells stimulated with LPS, the effect of which could be blocked by RU486. N = 4/group. One-way ANOVA with Dunnett’s multiple comparison test was used for the statistics. ***p < 0.001 vs LPS group; &p < 0.05 vs LPS + ASI group; ###p < 0.001 vs LPS + Dex group, Dex, dexamethasone. (B) GR siRNA could effectively inhibit its protein expression. (C) Reduction of GR by siRNA decreased the inhibitory effect of ASI on protein expression of iNOS in BV-2 cells upon LPS stimulation. (D) Inhibitory rate of ASI on iNOS protein expression was significantly lower in siGR transfected plus LPS-stimulated BV-2 cells than that in LPS-stimulated BV-2 cells. Un-paired t-test was used for the statistics. **p < 0.01 vs LPS + ASI group, N = 3/group, mean ± S.E.M. (E) ASI time-dependently abolished phosphorylation of PI3K and Akt in BV-2 cells upon LPS stimulation. (F) si GR abolished ASI mediated downregulation of phosphorylation of PI3K and Akt in LPS-stumulated BV-2 cells. The data shown were one of the representative results from at least three independent experiments.
