Figure 8
hGR and mGR’s SBDs are highly similar with each other in both sequence and 3D structure.
(A) Sequence alignment of hGR and mGR SBD. (B) 3D structure (PDB ID: 1M2Z) of GR SBD. The different color represents different secondary structures: red, alpha-helix; yellow, beta-sheet; grey, random coil or turn. Generally, it is composed of 16 rigid components, out of which 12 are alpha-helix and 4 are anti-beta-sheet. a: alpha-helix; b: beta-sheet. The number after the letter stands for the order in sequence, from N-terminal to C-terminal. (C) The superposition of hGR SBD and mGR SBD complex: blue, hGR SBD (PDB ID: 1M2Z); grey, mGR SBD (PDB ID: 3MNE). Those two structures bind to the same ligand, dexamethasone, which has also been shown in the graph. Both the protein and ligand are superposed very well with each other, the RMSD of protein CA is 1.17 Å.
