Figure 3
Transcriptional regulation of SF-1 by sumoylation and ubiquitination.
(a) Effects of SUMO and ubiquitin on the regulation of transcriptional activity of SF-1. HEK293 cells were transfected with CB1-Luc, BDNF-Luc, SF-1, SUMO, or ubiquitin as indicated and luciferase activities were determined (**P < 0.01 vs. SF-1 only). (b) Transcriptional activity of wild-type SF-1 or the K194R mutant under the control of nutritional conditions. The values are presented as the mean ± SEM from more than three independent experiments (***P < 0.001, **P < 0.01). (c) SF-1 binding on CB1 promoter is modulated by sumoylation and ubiquitination. Chromatin was extracted from Neuro2A cells expressing Flag-SF-1, SUMO, or ubiquitin and immunoprecipitated using anti-Flag antibody. (d) Binding of wild-type SF-1 or the K194R mutant on the CB1 promoter under different nutritional conditions. Neuro2A cells were transfected with Flag-tagged SF-1 constructs encoding wild-type or the K194R mutant and incubated in culture medium with or without serum for 6 hours.
