Figure 3

Summary of the effect of MPP⁺ on NADH fluorescence lifetime components.

Average of all the data points (n = 75) from five experimental days of NADH lifetime measurements: (a) short, or free (τ₁), (b) long, or protein-bound (τ₂), (c) average (τ_avg) NADH lifetimes and (d) the ratio of the relative contribution of free and protein-bound NADH (a₁/a₂) for cells treated with different concentrations of MPP⁺. The error bars indicate standard error of the mean (SEM). A one-way ANOVA with LSD-post-hoc analysis was performed to define statistical significance. Statistical significance: **p < 0.001, for control (0 μM MPP⁺) vs. MPP⁺ treated cells; #p < 0.05, ##p < 0.001, for 50 μM MPP⁺ treatment vs. other MPP⁺ treated cells; ^Δp < 0.05, ^ΔΔp < 0.001, for 100 μM MPP⁺ treatment vs. other MPP⁺ treated cells; ^◊p < 0.05, ^◊◊p < 0.001, for 250 μM MPP⁺ treatment vs. other MPP⁺ treated cells; ^●p < 0.05, ^●●p < 0.001, for 500 vs. 1000 μM MPP⁺ treatment.