Figure 4 | Scientific Reports

Figure 4

From: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus

Figure 4

Double fluorescent gene excision using Cre/Lox System.

(a) Procedure of double fluorescent gene excision by Cre/Lox system. HEK293T cells were transfected with Cre-recombinase (b) or control plasmids (c) and then infected with fluorescent gene recombinant virus (MOI = 1). The images were taken at 36 hours after infection. (d) Plaque purification for double fluorescent genes excision virus. Cell plaques were indicated by arrows.

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