Figure 5
From: Irx3 is required for postnatal maturation of the mouse ventricular conduction system
Irx3 physically interacts with Tbx5.
(a) Gene expression analysis showed a reduction in Cx40 (Gja5) expression, but increases in Nkx2.5 and Tbx5 expression in P4 Irx3−/− endomyocardium compared to controls. n = 5 for each group. *P < 0.05 vs. Irx3+/+ heart. (b) Adenovirus-mediated Irx3 overexpression (Ad-Irx3) in cultured neonatal ventricular myocytes resulted in elevated Cx40 expression, but no changes in Nkx2.5, Tbx5 and Id2 expression, compared to control cells infected with GFP-expressing adenovirus (Ad-GFP). n = 3 for each group. *P < 0.05 vs. Ad-GFP. (c) Co-immunoprecipitation assay demonstrated a direct physical interaction of Irx3 with Tbx5.
