Figure 7
From: Irx3 is required for postnatal maturation of the mouse ventricular conduction system
Irx3 promotes expression of VCS-enriched Nkx2.5 and Tbx5 target genes.
(a) Expression of selected VCS-enriched genes, shown as the log2 expression ratio between Purkinje fibers (PF) and working myocardium (WM). Gene are color-coded for Nkx2-5-Irx-VCS (green), Tbx5-Irx-VCS (red) and Nkx2.5-Tbx5-Irx-VCS (yellow). (b) qPCR experiment demonstrates that Irx3 activates most of VCS-enriched genes in cardiomyocytes, shown as the log2 expression ratio between Ad-Irx3 and Ad-GFP. n = 3–5 for each group. *P < 0.05 vs. Ad-GFP.
